It marks the biggest one-day rise since the outbreak was declared last August.
The outbreak of the haemorrhagic fever in eastern Congo is the second largest in history, having killed some 660 people and infected nearly 400 more.
Health workers are being hampered by rampant distrust and violence.
At least five treatment centres have been attacked by armed gangs.
And medical charities have suspended aid amid fears of further violence in the epicentre of the outbreak.
Ebola has a kill rate of around 50% – and is a hemorrhagic fever.
People are believed to contract the virus through close contact with infect people or animals.
It leads to people suffering fever and fatigue, before being struck with vomiting, diarrhoea and oozing blood.
Last month, health officials in South Sudan were pictured desperately preparing for the spread of the virus.
UNICEF workers carried out training exercises in Juba, the Republic’s capital.
Kitted out in biohazard suits, the workers moved dummies covered in fake blood as part of the session.