Howard Matthew Webb, 31, is accused of putting both his testicles in a customer’s salsa container while delivering food from a Mexican restaurant.
He was arrested on Friday after a 14-second clip emerged of him appearing to take the disgusting revenge.
The order was placed on January 12 on the online meal-ordering service Dinner Delivered, from a restaurant in Maryville, Tennessee.
Police said Webb was a passenger in the vehicle when the order was delivered, and the driver filmed the video.
In the clip, Webb is shown placing his testicles in the customer’s salsa.
The driver says: “This is what you get when you give an 89 cents tip for an almost 30-minute drive.”
Webb is then heard saying: “Oh, oh, it feels good on my balls,” according to an arrest warrant obtained by US media.
The clip went viral online, and Dinner Delivered soon notified authorities.
Webb was arrested and charged with adulteration of food.
“It feels good on my balls”
Howard Matthew Webb
He remains in jail on $45,000 (£34,000) bond.
His preliminary hearing was set for March 12.
If the jury finds him guilty of the crime — and proves he intended to cause “bodily injury” to someone else, he could face three to 15 years in prison and a fine of up to $10,000 (£7,500).
Dinner Delivered general manager Aliyah Wilson said the driver no longer worked for them and said Webb was not connected to the company.
“He will never drive for Dinner delivered again,” she told US media.
She said they had been able to track down the video due to the 89-cent tip remark.
The company has also reportedly issued a refund to the customer.
April M Pennington who lives in Maryville said she saw the footage through a Facebook group chat and reported it to Dinner Delivered before posting it online.