The festival gates don’t officially open until 8am – but many ticket-holders keen to get the party started have already begun the journey.
Up to 200,000 people from all over the UK and around the world are headed for the site in Pilton, Somerset.
Many have chosen to sleep in their cars and travel on overnight buses to ensure they beat the queues and find a good spot for camping.
Unable to contain their excitement, many festival-goers took to Twitter to spread the festival fever.
One wrote: “Off to Glastonbury, my favourite place on Earth. See you on the other side!”
“Glastonbury bound!!! Goodbye normal world””
Another added: Glastonbury bound!!! Goodbye normal world”.
Campers are due to be greeted by cloudy but dry weather when the gates open, with sunshine and temperatures of up to 23 degrees from mid-afternoon.
Organisers have urged those attending to bring their own reusable water bottles to the 900-acre site, as 2019 marks the first year that single-use plastic bottles have been banned.
The five-day event, the largest greenfield music and performing arts festival in the world, will be headlined by Stormzy, The Killers and The Cure.
Standard tickets for Glastonbury 2019 sold out in just 36 minutes.
Festival stalwart Norman Cook, aka Fatboy Slim, has played at Glastonbury since 1996 and will be performing three DJ sets this year.
He said: “Glastonbury is a town the size of Colchester, populated by a bunch of lunatics escaping from reality and escaping from convention.”
“For four days we get to live a fantasy, Utopian existence.”
Forecasters warned there could be severe storms during the latter part of the festival, with thunderstorms and potential flooding.
The Met Office has also urged those attending to take extra precautions such as sunscreen and to seek shelter from the sunshine due to high UV levels.
In 2017, the Wednesday of Glastonbury Festival was the hottest day in the event’s history, with temperatures hitting 31C and leading to dozen of people being treated by paramedics.
But, only eight years of the festival – including the first Glastonbury in 1970 – have not seen any rain.