The northbound carriageway has been shut between Junction 9 and Junction 11 following the collision at around 5.20am.
It will remain closed until tomorrow due to major damage to the road.
The southbound carriageway has been shut between Junction 9 and Junction 10.
Highways England tweeted: “The #M5 Northbound carriageway between J11 #Gloucester and J9 #Ashchurch will remain CLOSED until tomorrow due to extensive road surface damage that must be resurfaced as a result of this incident.
“Please plan ahead and consider different routes where possible to avoid the area.”
In an earlier tweet, Highways England said: “#M5 Northbound J10 #Cheltenham towards J9 #Tewkesbury. There has been an RTC.
“All emergency services are on route and emergency road closures will be installed from J11 #Gloucester.
“Both directions are now being closed for a serious RTC.
“Northbound closed between J11 #Gloucester and J9 #Tewkesbury. Southbound closed between J9 #Tewkesbury and J10 #Cheltenham.
“Diversions are being installed and closures will likely be in place through to the afternoon.”
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